Who is this test for?
People at high risk of not getting enough vitamin D:
- People with dark skin e.g. African, African-Caribbean or south Asian
- Children aged 0 to 4
- People who are not often outdoors
- People who are in an institution like a care home
- People who usually wear clothes that cover up their skin
What is in the test?
- You will require a timer
- Test cassette
- Instructions for use
- Colour card
- Plaster
- Alcohol wipe
- Lancet
- Buffer solution
- Capillary dropper
How do you perform the test?

Results

Deficient: Very low vitamin D levels
• Two lines appear: one in the control region (C) and one in the test region (T).
• The test line (T) is as dark or darker than the 10 ng/mL line on the provided colour card.

Insufficient: Low vitamin D levels
• Two lines appear: one in the control region (C) and one in the test region (T).
• The test line (T) is darker than the 30 ng/mL line but lighter than the 10 ng/mL line on the provided colour card.

Excess: High vitamin D levels
• Only one line appears in the control region (C).
• No line appears in the test region (T).

Invalid: Testing error. Repeat with a new test
The control line (C) fails to appear. Review the instructions and repeat with a new test kit.
Want to know more?
Vitamin D helps regulate the amount of calcium and phosphate in the body. These nutrients are needed to keep bones, teeth and muscles healthy. Vitamin D deficiency occurs when the body doesn’t get enough vitamin D from sunlight or diet. Vitamin D deficiency can cause loss of bone density, osteoporosis, broken bones and rickets in children.
Vitamin D is sometimes called the sunshine vitamin because your body makes it from cholesterol when your body is exposed to sunlight. During the autumn and winter, you need to get vitamin D from your diet because the sun is not strong enough for the body to make vitamin D.
But since it’s difficult for people to get enough vitamin D from food alone, the NHS recommends everyone (including pregnant and breastfeeding women) should consider taking a daily supplement containing 10 micrograms of vitamin D during the autumn and winter.
Between late March to the end of September, most people can make all the vitamin D they need through sunlight on their skin and from a balanced diet.
Before your body can use vitamin D, your liver must change it into another form called 25 hydroxyvitamin D, or 25(OH)D. The Vitamin D Test measures the level of 25(OH)D in your blood in order to determine whether your body has deficient, insufficient, sufficient or excess levels of vitamin D in your blood to enable/prevent your body from working well.
Doctor approved